A role for the yeast PCNA unloader Elg1 in eliciting the

A role for the yeast PCNA unloader Elg1 in eliciting the

A role for the yeast PCNA unloader Elg1 in eliciting the

The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA during DNA damage. Using DNA damage checkpoint (DC)-inducible and replication checkpoint (RC)-inducible strains, we show that Elg1 is essential for eliciting the …

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Is PCNA unloading the central function of the Elg1/ATAD5

PCNA Unloading by S. cerevisiae Elg1-RLC Several lines of evidence from yeast suggested a role for Elg1-RLC in PCNA regulation during DNA replication.6,29,36 Martin Kupiec's group demonstrated that yeast Elg1-RLC preferentially interact with SUMOylated PCNA, and in cells lacking Elg1, they found SUMOylated PCNA accumulated on chromatin in

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The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role

Dec 18, 2020 · In cells, each complex loads PCNA for different purposes; RFC-loaded PCNA is essential for DNA replication, while CTF18-RLC-loaded PCNA participates in cohesion establishment and checkpoint activation. After completing its tasks, PCNA is unloaded by ATAD5 (Elg1 in yeast)-RLC.

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The PCNA-RFC families of DNA clamps and clamp loaders

A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint. 2019) uncovered a role for Elg1 in eliciting the DNA damage checkpoint (DC), one of …

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Eukaryotic clamp loaders and unloaders in the maintenance

May 31, 2019 · To investigate whether RTS1-induced recombination depends on the PCNA unloader Elg1, we compared recombination frequencies in wild-type and elg1∆ strains harbouring either RTS1-IO or -AO (Figure 1C,D).In line with the observation that an elg1∆ mutant exhibits increased spontaneous direct repeat recombination in budding yeast (Gazy et al., 2013), a …

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Division of Labor between PCNA Loaders in DNA Replication

May 21, 2020 · PCNA recycling, following completion of Okazaki fragment maturation, is thought to be the task of Elg1-RFC, a specialized PCNA unloader (Kang et al., 2019, Kubota et al., 2013). Ctf18-RFC is unique in that Ctf18 comes with two additional subunits, Ctf8 and Dcc1 ( …

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How yeast cells deal with stalled replication forks

May 11, 2020 · It is clear that the role of Srs2 is far from been completely understood and that we are looking through a keyhole, each time gazing at a different part of its activity but still lacking the ability to see the big picture. Sau S, Kupiec M (2020) A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint. Curr Genet 66:79

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The PCNA unloader Elg1 promotes recombination at collapsed

May 31, 2019 · To investigate whether RTS1-induced recombination depends on the PCNA unloader Elg1, we compared recombination frequencies in wild-type and elg1∆ strains harbouring either RTS1-IO or -AO (Figure 1C,D).In line with the observation that an elg1∆ mutant exhibits increased spontaneous direct repeat recombination in budding yeast (Gazy et al., 2013), a …

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A role for the yeast PCNA unloader Elg1 in eliciting the

IMPORTANCE The Elg1protein forms an RFC-like complex in charge of unloading PCNA from chromatin during DNA replication and repair. Mutations in the ELG1 gene caused genomic instability in all organisms tested and cancer in mammals. Here we show that Elg1 plays a role in the induction of the DNA damage check-

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The PCNA-RFC families of DNA clamps and clamp loaders

A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint. 2019) uncovered a role for Elg1 in eliciting the DNA damage checkpoint (DC), one of …

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structure–function analysis of the yeast Elg1 protein

The yeast Elg1 interacts both genetically and physically with the yeast orthologs of the Fanconi Anemia repair pathway . Although it has already been established that Elg1 is an unloader of PCNA, it is still not clear whether the phenotypes of elg1Δ cells are a consequence of PCNA retention on the chromatin. To address this issue, we have used

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The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role

Up to10%cash back · Jul 22, 2019 · During cell proliferation, the genome is constantly threatened by cellular and external factors. When the DNA is damaged, or when its faithful duplication is delayed by DNA polymerase stalling, the cells induce a coordinated response termed the DNA damage response (DDR) or checkpoint. Elg1 forms an RFC-like complex in …

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Publications | mklab

Elg1 forms an RFC-like complex in charge of unloading the DNA polymerase processively factor PCNA during DNA replication and DNA repair. Using checkpoint-inducible strains, a recently published paper (Sau et al. in mBio 10(3):e01159-19. https://doi.org/10.1128/mbio.01159-19, 2019) uncovered a role for Elg1 in eliciting the DNA damage checkpoint (DC), one of the …

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Eukaryotic clamp loaders and unloaders in the maintenance

Dec 18, 2020 · PCNA unloading. After completion of DNA synthesis during DNA replication and repair, PCNA is unloaded from DNA by the eukaryotic clamp unloader ATAD5-RLC (Elg1-RLC in yeast) 10,16,17,30.The RFC complex can unload PCNA in vitro 9, even though its activity is significantly lower than that of ATAD5-RLC 10.It has been reported that yeast Ctf18-RLC can …

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Research Publication

Telomeres and stress in yeast cells: When genes and environment interact. A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint. Role of Digital Divide in the Effectiveness of e-governance: A Study on Rayagada District of Odisha .

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Replication-Coupled PCNA Unloading by the Elg1 Complex

function of S. cerevisiae Elg1-RLC is unloading of PCNA during DNA replication (Kubota et al., 2013a, 2013b). The function of the Elg1-RLC in PCNA unloading appears to be conserved in humans, since ATAD5 (the human Elg1 homolog) is required for proper removal of PCNA from chromatin in human cell lines (Lee et al., 2013; Shiomi and Nishitani, 2013).

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Elg1, a central player in genome stability - ScienceDirect

Jan 01, 2015 · Taken together, these results suggest a role for the Elg1-RLC in PCNA unloading, possibly during DNA replication, but more likely as a response to fork stalling or collapse. As most forms of DNA repair and damage bypass require DNA synthesis, PCNA participates in repair, and needs to be loaded and unloaded there too [64] .

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Quantitative proteomic analysis of yeast DNA replication

A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint relationship between the function of Elg1/ATAD5-RLC in PCNA unloading and the …

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Quantitative proteomic analysis of yeast DNA replication

A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint relationship between the function of Elg1/ATAD5-RLC in PCNA unloading and the …

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A role for the yeast PCNA unloader Elg1 in eliciting the

Jul 22, 2019 · Elg1 forms an RFC-like complex in charge of unloading the DNA polymerase processively factor PCNA during DNA replication and DNA repair. Using checkpoint-inducible strains, a recently published paper (Sau et al. in mBio 10(3):e01159-19. https://doi.org/10.1128/mbio.01159-19, 2019) uncovered a role for Elg1 in eliciting the DNA …

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